Search result for 'flower '.
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N16332
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Name: hapless 28 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
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Locus
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Stock type: individual line
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Material type: seed
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Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, reduced transmission through the male gametophyte (MG), does not appreciably affect the female gametophyte (FG); strong, but not complete impact on pollen function; short pollen tube growth, failing to exit the style.
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N16333
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Name: hapless 29 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
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Locus
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Stock type: individual line
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Material type: seed
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Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, reduced transmission through the female gametophyte (FG); nearly completely penetrant; no obvious defect in pollen grain development, pollen tube growth through the style and pollen tube growth or guidance in the ovary.
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N16334
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Name: hapless 30 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
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Locus
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Stock type: individual line
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Material type: seed
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Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, affecting the male gametophyte (MG) and the female gametophyte (FG), complete elimination of the function of the FG while having a mild impact on MG function; pollen tube growth path appears normal, yet tubes fail to enter the micropyle.
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N16335
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Name: hapless 31 |
Price:
£11.00
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Donor
- Brown University Mark Johnson
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Locus
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Stock type: individual line
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Material type: seed
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Description
Line generated in the qrt1-1 background by Agrobacterium-mediated transformation with the pCSA110 T-DNA clone encoding GUS under the control of the postmeiotic, pollen-specific LAT52 promoter, as well as resistance to the herbicide Basta (Glufosinate). Individual BastaR transgenic plants (primary transformants, T1) were self-fertilized to yield T2 seeds. T2 seeds were plated on MS medium containing 50 mg/liter Basta and the percentage of BastaR seedlings was determined. One stage 14 flower from each BastaR plant retained was fixed and stained in X-Gluc overnight at 37ø. Pollen tetrads were assayed for meiotic segregation of the LAT52:GUS gene using an inverted microscope. Transmission of the T-DNA through the male gametophyte was tested by pollinating stage 14 ms1 flowers (male-sterile, Ler ecotype) with mature pollen from hap+/- plants. Transmission through the female gametophyte was monitored by emasculating stage 12 hap+/- flowers and pollinating them 24-40 hr later with qrt1-1 (Col ecotype) pollen. F1 seed was plated on Basta-containing MS media and seedlings were scored for resistance or sensitivity. Pollen behavior was examined after crossing three plants from each hap line to three or more ms1 pistils and allowing 12 hr for pollen tube growth. Pistils were excised and mounted on double-sided tape; ovary walls were then removed under a dissecting scope. Pistils were prepared and imaged using a Zeiss Axioskop. For closer examination of pollen tube behavior on the ovule, pistils were stained in 0.1% Congo red following incubation in X-Gluc, allowing analysis by confocal laser scanning microscopy. Genomic sequences flanking the right and left T-DNA borders were amplified with thermal asymmetric interlaced (TAIL) PCR.
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Phenotype
Distorted segregation, reduced transmission through the male gametophyte, does not appreciably affect the female gametophyte; pollen tube growth in ovary is short, but pollen tubes target the ovules they reach.
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N16357
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Name: GA3ox2-TC-GUS |
Price:
£11.00
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Donor
- Duke University Tai-ping Sun
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Stock type: individual line
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Material type: seed
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Description
Transgenic GUS-fusion expression line # 3-2-5, harboring the GA3ox2-TC-GUS construct. The promoter- á -glucuronidase (GUS) transcriptional construct (TC-GUS) was generated using a 3 kb sequence upstream of GA3ox2; final construct in Agrobacterium was transformed into Columbia ecotype plants by using the floral dip method; transformed plants were selected with kanamycin.
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Phenotype
Strong expression in vegetative tissues; weak expression in anthers during flower development.
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N16486
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Name: dvl1-1D |
Price:
£11.00
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Donor
- University of Missouri John Walker
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Locus
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Stock type: individual line
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Material type: seed
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Description
Line isolated by screening T-DNA activation tagged lines generated in er-116, a weak allele of ERECTA. By inverse PCR, the T-DNA insertion was found to be 1482 bp downstream of the stop codon of At5g16020 with the enhancer elements towards the gene At5g16020.
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Phenotype
Small rosettes with round leaves and short petioles; clustered inflorescences with short flower buds; short sepals and petals; short pedicels and siliques; filaments and stamens are able to extend to reach the stigma, consequently plants are fully fertile; the two valves of the silique do not taper apically, the ends are broadened with four horn-like protrusions at the fruit tip; short plants (about 70% of the height of wild-type plants).
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N22224
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Name: CIBC-5 |
Price:
£11.00
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Donor
- University of Chicago Joy Bergelson
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Stock type: individual line
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Material type: seed
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Phenotype
large rosettes with numerous leaves, large leaves with elongated petiols, leaf margins serrated, late flowering, numerous axillary inflorescences, height= 38-43cm.
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N22236
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Name: CIBC-17 |
Price:
£11.00
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Donor
- University of Chicago Joy Bergelson
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Stock type: individual line
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Material type: seed
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Phenotype
large and flat rosettes with numerous leaves, large and wide leaves, late flowering, numerous axillary inflorescences, height=35-50 cm.
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N22504
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Name: Thellungiella halophila |
Price:
£11.00
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Donor
- Purdue University Ray Bressan
- University of California, Riverside Jian-Kang Zhu
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Stock type: individual line
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Material type: seed
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Phenotype
Common name: salt cress, close relative of Arabidopsis thaliana (Brassica familiy); extremely salt tolerant; life cycle, size, development, and structure resembles Arabidopsis, but compared with Arabidopsis (Columbia ecotype) there are several differences: a) leaves are more elongated and serrated, with longer petioles; b) rosettes have obligate vernalization requirement (minimun 3 weeks) in order to flower; c) all stamens are of equal length, whereas two different length classes are found in Arabidopsis; d) self-fertile and continues flowering later than Arabidopsis; e) has slightly shorter siliques than Arabidopsis; f) seeds are slightly more elongated; g) plants are able to withstand dramatic salinity shock up to 500 mM NaCl and grow in salt far in excess of the capability of Arabidopsis; h) has 7 chromosomes. It cannot be crossed successfully with Arabidopsis despite having been considered synonymous with Arabidopsis in the past (Al-Shehbaz and O'Kane, 1995; Al-Shehbaz et al., 1999); EST analyses of several hundred salt cress clones revealed averages of 90% and 95% identities between salt cress and Arabidopsis cDNA and amino acid sequences, respectively.
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N22586
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Name: Ull2-5 |
Price:
£11.00
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Donor
- University of Southern California Magnus Nordborg
- University of Chicago Martin Kreitman
- University of Chicago Joy Bergelson
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Locus
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Photo
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Stock type: individual line
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Material type: seed
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Phenotype
Large rosettes with numerous leaves, leaf margins serrated, late flowering (vernalization at rosette stage may promote earlier flowering), dark -green plants, height= 42-48 cm. Plants did flower after 4 wk vernalization but considerably later than H51. Shindo, et al. (2006). Despite responding to 4 wk of cold, Var-2-6 and Ull-2-5 still flowered late after >6 wk of cold; i.e., the vernalization response was relatively slow.Flowered extremely late after 8 wk vernalization and required at least 14 wk of cold to fully saturate the vernalization requirement. FLC levels decreased during cold treatment but then increased significantly after the plants were returned to 23°C.After 4 wk vernalization, FLC levels had increased (by 30 d after transfer to 23°C) to the same level as nonvernalized plants. After 6 wk of cold, FLC levels were lower than those observed at 4 wk.
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